PPT Slide
[1385-P] Metabolic Differences Following Pre-Hepatic Versus Systemic Administration of Insulin in Diabetic Dogs E. JOACHIM FREYSE, UWE FISCHER, SIEGFRIED KNOSPE, G. C. FORD, K. SREEKUMARAN NAIR Karlsburg, Germany; Heringsdorf, Germany; Rochester, MN Pre-hepatic insulin (PI) delivery in normal physiology provides more than two-fold higher insulin levels in hepatic than in the systemic circulation. In contrast, peripheral insulin (SI) administration in diabetic patients results in similar in hepatic and systemic insulin levels. We investigated the potential differences in energy and protein metabolism in ketosis prone diabetic dogs between PI and SI infusion while maintaining similar glucose levels. Eight diabetic dogs (26±5 months, 23±1 kg, diabetic for 5±3 months) were infused with PI and SI in paired experiments. To maintain normoglycemia (5.1±0.3 vs. 5.5±0.3 mmol/l; NS) more insulin was infused in PI than in SI (54±4 vs. 27±2 mU·kg-1·h-1; pɘ.05). Plasma insulin, however, tended to be lower with PI than with SI (114±10 vs. 139±9 pmol/l). Glucose kinetics were identical in both groups. Oxygen consumption (PI:161±8 Vs SI:130±13 ml/min; pɘ.05), CO2 production (130±8 ml/min vs. 99±10 ; pɘ.05), RQ (0.81±0.01 vs. 0.76±0.02; pɘ.05) and EE (1089±60 vs. 870±90 kcal/24 h; pɘ.05) were lower in SI. In addition leucine oxidation (PI:55±5Vs SI:36±4µmol·kg-1·h-1; pɘ.05), Fractional synthesis rates of albumin (PI:0.68±0.11%/hVs SI: 0.55±0.15, Pɘ.02), fibrinogen (2.6±0.6 Vs1.7±0.5, Pɘ.03), and liver tissue protein (0.78±0.6 vs. 0.68±0.5 %/h; pɘ.05) were lower when insulin was administered systemically. Systemic insulin administration results in reduced energy expenditure, synthetic rates of several liver proteins and leucine oxidation, all of which account for differences in energy expenditure. The reduced energy expenditure in association with systemic insulin administration may promote weight gain that is often a problem in diabetic patients on insulin treatment. Sunday, June 06, 2004 12:00 PM Poster Presentation: 18 A - Integrated Physiology - Amino Acid Metabolism (12:00 PM - 2:00 PM)